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Chaque projet soutenu par Gebert Rüf Stiftung est présenté sur le site web de la fondation avec en particulier les données de base du projet. Par cette publication, la fondation informe sur les résultats du soutien accordé et contribue à la communication scientifique au sein de la société.


Nematrix Technologies


The project management is responsible for the content of the information provided.


This project, funded by Gebert Rüf Stiftung, is supported by the following project partners: EPFL, Laboratory of Microsystems (LMIS2); EPFL, Laboratory of Integrative Systems Physiology (LISP); Swiss Laboratory Animal Science Association (SGV)

Données de projet

  • Numéro du projet: GRS-025/16 
  • Subside accordé: CHF 300'000 
  • Consentement: 02.11.2016 
  • Durée: 01.2017 - 09.2018 
  • Champs d'activité:  Pilotprojekte, 1998 - 2018

Direction du projet

Description du projet

To cure the relentless decline of the drug discovery industry efficiency, a radical change seems today to be needed. Recently, alternative biological models to standard cell cultures and rodents started proving their potential to revolutionize the field. While 3-dimensional cell cultures will likely replace their flat homologous in the near future, small model organisms, such as the tiny roundworm Caenorhabditis elegans, are gaining increasing attention as valid alternatives to mice in various drug screening contexts. A widespread adoption of these models would indeed significantly lower both time and costs of pharmaceutical research and improve its efficiency, by reducing mice testing and optimizing the analysis pipeline in the pre-clinical phase. Unfortunately, however, the protocols for culture and study of these alternative models still mainly rely on the manual techniques currently used in academic biological research, which definitely lack the reproducibility and throughput standards required by the drug discovery industry. To enable such change, a team of scientists at the École Polytechnique Fédérale de Lausanne (EPFL) is working at the interface between microtechnologies and life sciences to develop the first automated platform for roundworm culture, analysis and drug testing. Our technology employs microfluidics -the accurate manipulation of fluids at the sub-millimeter scale- for the precise and automated handling of roundworms and reagents within miniaturized devices. This offers unprecedented control over both worm culture and drug screening conditions, while allowing real-time monitoring of full sets of worm analysis parameters, in a high-throughput format. Overall, we aim to create the first “organism-on-chip” technology for drug screening and pave the way for the widespread use of roundworms -and, possibly, of other small model organisms- for the replacement, reduction and refinement of animal testing within the drug discovery industry.

Quelles sont les particularités de ce projet?

Our project aims to create a new technology capable of generating complete multi-phenotypic profiles of worms over long-term experiments, without human intervention. From a technological viewpoint, the key element of our innovation consists in the use of passive hydrodynamics for the manipulation and study of roundworms within microfluidic cartridges. In particular, the use of microfluidics ensures: protocol automation, standardization and scalability towards high-throughput applications; (i) fast and accurate regulation of effective drug concentrations within our devices; (ii) more efficient drug intake with respect to currently used culture conditions and minimal consumption of expensive reagents. Our technology is moreover expected to open unprecedented possibilities in terms of spatio-temporal accuracy of the drug tests.

Etat/résultats intermédiaires

We validated the scientific value of microfluidics as a tool to improve C. elegans research, by demonstrating the first microfluidic chip for high-throughput analysis of C. elegans embryos as well as a semi-automated microfluidic-based device for long-term studies on neurodegenerative disease worm models. We developed a new class of microfluidic devices for the controlled growth and culture of C. elegans throughout their full life-cycle, together with the first version of a laboratory prototype devoted to the automated handling of drug screening experiments on these devices. We pursued biological validation studies to corroborate the value of worm models within different fields of application, with a specific focus on anthelmintic drug discovery. We are currently engaged in a new series of pilot studies with industrial collaborators, aiming to define clear assay formats for their internal research and promote the adoption of our innovative technology within the pharmaceutical domain in the near future.

Revue de presse


Personnes participant au projet

Matteo Cornaglia, project leader, PhD Microtechnology, EPFL
Laurent Mouchiroud, PhD biology, Lyon
Fabien Tâche, PhD robotics, ETH
Aurélien Ponzio, MS Microtechnology, EPFL
Sumegha Mantri, MA product design, ECAL
Victor Moynier, MA product design, ECAL
Joëlle Aeschlimann, MA UI/UX design, ECAL

Prof. Dr. Martin Gijs, PI at EPFL/LMIS2
Prof. Dr. med. Johan Auwerx, PI at EPFL/LISP
Prof. Lluis Fajas Coll, PI at UNIL/CIG

Dernière mise à jour de cette présentation du projet  25.11.2019